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  • Title: Metabolic changes in Crithidia fasciculata accompanying physiological adaptation to growth in the presence of carbonyl cyanide m-chlorophenylhydrazone.
    Author: Kutzman RS, Roberts JF.
    Journal: Comp Biochem Physiol B; 1979; 62(4):449-53. PubMed ID: 45556.
    Abstract:
    1. Crithidia fasciculata adapted to growth in the presence of 10(-5) M carbonyl cyanide m-chlorophenylhydrazone (CCCP), an uncoupler of oxidative phosphorylation, maintained adenosine phosphate pools and an adenylate energy charge comparable to those of control cells. 2. CCCP-adapted cells in the presence of the uncoupler respire endogenous substrate at a greater rate than control cells and this effect of CCCP appears readily reversible. 3. CCCP-treated, adapted cells, supporting high endogenous respiration rates, were not responsive to added substrates which significantly stimulated the oxygen utilization of normal C. fasciculata. 4. CCCP-adapted cells, provided with [U-14C]-labeled proline, utilize this substrate at 67% the rate of control cells, but divide the isotopic label between CO2 and protein in a ratio identical to that of normal cells. 5. The transport of alanine and proline by adapted C. fasciculata was severely impaired, while the transport of tyrosine and leucine was unaffected.
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