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  • Title: Recognition of nonsense codons in mammalian cells.
    Author: Hatfield D.
    Journal: Proc Natl Acad Sci U S A; 1972 Oct; 69(10):3014-8. PubMed ID: 4562751.
    Abstract:
    The tritiated trinucleotide UGA was used in a binding assay to detect transfer RNAs that recognize this nonsense codon from calf-liver cells. Acylation of transfer RNA with labeled amino acids and determination of codon responses of aminoacyl-tRNAs demonstrate that a species of seryl-tRNA and a species of arginyl-tRNA recognize the codon UGA. Slight responses of cysteinyl-tRNA and of trytophanyl-tRNA to the codon UGA were also observed.
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