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  • Title: Temperature-sensitive mutants of Escherichia coli requiring saturated and unsaturated fatty acids for growth: isolation and properties.
    Author: Harder ME, Beacham IR, Cronan JE, Beacham K, Honegger JL, Silbert DF.
    Journal: Proc Natl Acad Sci U S A; 1972 Nov; 69(11):3105-9. PubMed ID: 4564200.
    Abstract:
    A procedure is described for selection of temperature-sensitive mutants affecting fatty-acid synthesis based upon radiation suicide of wild-type organisms by tritiated acetate selectively incorporated into fatty acids. At 37 degrees , two of the mutants extensively incorporate fatty-acid supplements provided in the medium, and grow for extended periods only when a trans-unsaturated or a combination of saturated and cis-unsaturated fatty acids is available. In vivo fatty-acid synthesis, measured by [(14)C]acetate incorporation, is temperature-sensitive in these strains relative to protein synthesis and other non-lipid macromolecular syntheses using acetate. The biochemical nature of these mutations has not been identified.
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