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  • Title: Isolation of temperature-sensitive membrane mutants of Escherichia coli K-12.
    Author: Steenbakkers JF, Broekman JH, Kerkenaar A, de Haan PG.
    Journal: J Bacteriol; 1973 Nov; 116(2):535-40. PubMed ID: 4583239.
    Abstract:
    Mutants with impaired biosynthesis of unsaturated fatty acids or altered metabolism of the phospholipids were isolated at a rather high frequency from a set of temperature-sensitive lysis mutants. It is suggested that preselection for the lysis phenotype makes it possible to isolate several kinds of mutants affected in the integrity of the cytoplasmic membrane.
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