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  • Title: Isolation of Hfr strains from R+ and ColV2+ strains of Escherichia coli and derivation of an R'lac factor by transduction.
    Author: Nishimura A, Nishimura Y, Caro L.
    Journal: J Bacteriol; 1973 Dec; 116(3):1107-12. PubMed ID: 4584797.
    Abstract:
    Temperature-resistant revertants were isolated from Escherichia coli strains carrying a temperature-sensitive dnaA mutation (initiation of chromosome replication) and either a repressed or a derepressed F-like R factor or a ColV2 factor. Many of the revertants had all the properties of Hfr strains, with a variety of directions and origins of transfer. From one such revertant, episomes carrying the R factor and part of the lac region (R'lac) could be isolated by transduction. This system offers a good selection for Hfr strains produced by integration of various episomes and for the isolation of R' factors.
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