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  • Title: Some improved methods in P22 transduction.
    Author: Hartman PE.
    Journal: Genetics; 1974 Apr; 76(4):625-31. PubMed ID: 4599954.
    Abstract:
    Recent refinements simplify methods for P22 transduction in Salmonella and allow improved recovery of phage-free transductional clones. The methods include use of: integration- and lysis-defective phage mutants, heat-killed bacteria to eliminate free phage, direct plating of phage and bacteria, replica-plating for detection of phage content of individual clones, improved broth for phage growth, and procurement of high titer phage from P22 lysogens.
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