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  • Title: A study of clonal transfer in rabbits immunized with Salmonella abortus-equi.
    Author: Bordenave G.
    Journal: Immunology; 1974 Jul; 27(1):11-26. PubMed ID: 4604040.
    Abstract:
    We have attempted to reconstitute 750 R-irradiated New Zealand white rabbits with lymphoid cells from a donor immunized with Salmonella abortus-equi (S.a-e). Antibodies to S.a-e in the serum of such reconstituted recipients, have been principally studied by the IEF technique. Lymph node or bone marrow cells failed to reconstitute the irradiated recipients which began to produce antibodies to S.a-e as they recovered from irradiation (from 2 to 3 weeks after radiation exposure). Attempts made with spleen cells were sufficiently successful to permit the detection, during 3 weeks after cell transfer, of donor allotypes and donor idiotypes in recipient sera. One week after cell transfer, the pI spectra of antibodies to S.a-e in these sera were very heterogeneous and showed the same patterns of bands on IEF as the donor antibodies. The two or three clonal antibodies recognized by the anti-idiotypic serum did not segregate in the various recipients. This heterogeneity must be the reflection of the large number of spleen cells (2.5 × 107–1 × 108) we were obliged to use in order to find suitable amounts of donor idiotypes in recipient sera. As the rabbits were not inbred, donor cells were finally rejected by the hosts, 3 or 4 weeks after cell transfer. With such a system, selection of antibody-forming cell clones is a rare event. We have observed two cases out of a total of about hundred rabbits studied. These successful selections of restricted responses occurred in two out of four of the recipients of 5 × 106 donor spleen cells. When dominance of a strong clone was established, rejection of donor cells seemed to be delayed (from 3 to 5 weeks). With these outbred rabbits, it was not possible to propagate the selected clones further than a second transfer generation. Antibodies to S.a-e produced by irradiated rabbits used as controls (they received bacteria alone) showed, at the beginning of the recovery from irradiation (from 1 to 2 weeks after radiation exposure), very simple pI spectra as observed with neonatal rabbits during early steps of ontogeny of the antibody diversity.
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