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  • Title: Cell-free synthesis of a specific lipoprotein of the Escherichia coli outer membrane directed by purified messenger RNA.
    Author: Hirashima A, Wang S, Inouye M.
    Journal: Proc Natl Acad Sci U S A; 1974 Oct; 71(10):4149-53. PubMed ID: 4610570.
    Abstract:
    A specific lipoprotein of the E. coli outer membrane has been synthesized in a cell-free system directed by purified messenger RNA. The mRNA for the lipoprotein was purified as 7S RNA about 250-fold from exponentially growing cells. Protein synthesis of the cell-free system was totally dependent upon the addition of the purified mRNA. The product of the cell-free system was identified as the specific lipoprotein by immunoprecipitation and by peptide mapping.
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