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  • Title: Determination of the absolute number of Escherichia coli membrane vesicles that catalyze active transport.
    Author: Short SA, Kaback HR, Kaczorowski G, Fisher J, Walsh CT, Silverstein SC.
    Journal: Proc Natl Acad Sci U S A; 1974 Dec; 71(12):5032-6. PubMed ID: 4612538.
    Abstract:
    Transport of vinylglycolate (2-hydroxy-3-butenoic acid) via the lactate transport system is the limiting step for covalent labeling of membrane vesicles prepared from E. coli ML 308-225. Thus, the rate and extent of vinylglycolate labeling is stimulated about 10-fold by ascorbate-phenazine methosulfate, and stimulation is abolished by 2,4-dinitrophenol and by phospholipase treatment, neither of which affect the rate of vinylglycolate oxidation. [(3)H]Vinylglycolate of high specific activity has been prepared, and vesicles have been labeled with this compound in the presence of ascorbate-phenazine methosulfate. Examination of these preparations by high resolution radioautography in the electron microscope demonstrates that virtually all of the vesicles are labeled. The experiments provide a strong indication that most, if not all, of the membrane vesicles in these preparations catalyze active transport.
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