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  • Title: Nature of ribonucleic acid synthesis during early sporulation in Saccharomyces cerevisiae.
    Author: Chaffin WL, Sogin SJ, Halvorson HO.
    Journal: J Bacteriol; 1974 Nov; 120(2):872-9. PubMed ID: 4616951.
    Abstract:
    Phosphate uptake in sporulating cultures of Saccharomyces cerevisiae has been found to occur approximately 2 h after the transfer to sporulation medium. Early ribonucleic acid synthesis begins at approximately 4 h and continues to 8 h. Incorporation of phosphate into acid-extractable precursor pools parallels phosphate uptake. In triple-labeling experiments it was observed that the breakdown of vegetatively synthesized ribonucleic acid is not a significant source of precursors for ribonucleic acid synthesis during sporulation. The majority of the ribonucleic acid made in a 10-min period during sporulation does not migrate on gels with precursor or mature ribosomal ribonucleic acid.
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