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  • Title: Manipulative and inherent errors in anti-D quantitation using the AutoAnalyzer.
    Author: Gunson HH, Phillips PK, Stratton F.
    Journal: J Clin Pathol; 1972 Mar; 25(3):198-205. PubMed ID: 4622996.
    Abstract:
    The limits of accuracy for the quantitation of anti-D using the AutoAnalyzer have been reduced from +/- 26% with manually prepared dilutions to +/- 14% using an automatic pipette, as expressed by the 95% confidence limits. The error inherent in the AutoAnalyzer was estimated to contribute +/- 10% to the overall error. Problems associated with the reproducibility of this method for anti-D quantitation have been investigated, namely, the effect of the age of a given test cell, the use of different test cells, loss of sensitivity of the machine over a given day, and the reproducibility of results obtained at different positions on the standard graph.
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