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  • Title: Hyperprotease-producing mutants of Bacillus subtilis.
    Author: Higerd TB, Hoch JA, Spizizen J.
    Journal: J Bacteriol; 1972 Nov; 112(2):1026-8. PubMed ID: 4628743.
    Abstract:
    A number of mutants of Bacillus subtilis producing high levels of extracellular protease have been isolated. Analysis of culture supernatants of these mutants has shown that the total amount of proteolytic activity is elevated from 16- to 37-fold over the wild strain. The elevated activity was due to a simultaneous increase in both the neutral and alkaline protease. All of the mutants genetically analyzed were found linked to the argC4 marker by PBS-1 transduction analysis.
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