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  • Title: In vitro repair of x-irradiated DNA extracted from Bacillus subtilis deficient in polymerase I.
    Author: Laipis PJ, Ganesan AT.
    Journal: Proc Natl Acad Sci U S A; 1972 Nov; 69(11):3211-4. PubMed ID: 4628873.
    Abstract:
    DNA extracted from x-irradiated cells of a mutant of Bacillus subtilis deficient in DNA polymerase I has greatly reduced biological activity. This DNA, which has many single-strand nicks, can be repaired in vitro with purified DNA polymerase I and DNA ligase or with lysates of wild-type B. subtilis cells, which restore biological activity and increase the single-strand molecular weight. Lysates of polymerase I-deficient B. subtilis cells cannot repair such irradiated DNA until purified DNA polymerase I or lysate from wild-type cells is added to the deficient lysate.
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