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  • Title: [Denaturation of beef liver glutamate dehydrogenase under the action of guanidine hydrochloride and a study of the possibility of the enzyme renaturation].
    Author: Sugrobova NP, Gurevich VM, Chebotareva NA, Kurganov BI.
    Journal: Biokhimiia; 1979 Mar; 44(3):424-31. PubMed ID: 465590.
    Abstract:
    It was shown that denaturation of beef liver glutamate dehydrogenase under the action of guanidine hydrochloride results in a diplacement of the protein fluorescence maximum from 332 to 349 nm, in a decrease of optical rotation of the protein at 233 nm and in an appearance of negative bands in the difference absorbance spectrum with extrema at 279 and 287 nm. The transition of native enzyme into a denaturated state is observed within a narrow interval of guanidine hydrochloride concentrations. The middle point of the transition corresponds to approximately 2,2 M guanidine hydrochloride. The inactivation kinetics for glutamate dehydrogenase coincide with those of the enzyme spectral properties alterations due to denaturation. The attempts at renaturation of glutamate dehydrogenase by diluting the denaturated enzyme solution or by a dialysis against a buffer solution were unsuccessful.
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