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  • Title: Sectrofluorimetric micromethod for determining riboflavin in the blood of new-born babies and their mothers.
    Author: Knobloch E, Hodr R, Janda J, Herzmann J, Houdková V.
    Journal: Int J Vitam Nutr Res; 1979; 49(2):144-51. PubMed ID: 468469.
    Abstract:
    A microdetermination of riboflavin on the blood of new-born babies and their mothers was developed on the basis of hydrolysis of the blood in a trichloroacetic acid medium, separation of riboflavin and FMN on a Florisil column and measurement of the content spectrofluorimetrically by the standard addition method after elution with collidine buffer. The sensitivity of the method is 0.01 microgram/ml, the blood sample size 0.5--1.0 ml. The normal level in new-born babies in the first day of life was found to have an average value of 17.1+/-2.4 microgram/100 ml, in women 14.2+/-2.5 microgram/100 ml; the contents in cord blood and maternal vein blood were also determined.
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