These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Synthesis of the pyrrole porphobilinogen by sepharose-linked -aminolevulinic acid dehydratase.
    Author: Gurne D, Shemin D.
    Journal: Science; 1973 Jun 15; 180(4091):1188-90. PubMed ID: 4707065.
    Abstract:
    delta-Aminolevulinic acid dehydratase from Rhodopseudomonas spheroides was covalently linked to Sepharose 4B, which had been activated with cyanogen bromide. A column containing this enzyme gel readily catalyzed the synthesis of the pyrrole porphobilinogen on continuous passage of a solution of delta-aminolevulinic acid. Under the conditions of the procedures, product inhibition was minimized and a 50 to 94 percent yield was attained. A column containing about 1 milligram of enzyme was continuously operated for 27 days. Although its total activity appeared to be reduced about 30 percent at the end of this time, the bound enzyme produced approximately 200 milligrams of porphobilinogen each day, and about 5 grams of the pyrrole were isolated.
    [Abstract] [Full Text] [Related] [New Search]