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  • Title: Full length and discrete partial reverse transcripts of globin and chorion mRNAs.
    Author: Efstratiadis A, Maniatis T, Kafatos FC, Jeffrey A, Vournakis JN.
    Journal: Cell; 1975 Apr; 4(4):367-78. PubMed ID: 47272.
    Abstract:
    Rabbit globin mRNA was copied by AMV reverse transcriptase in the presence of various concentrations of deoxyribonucleotides (dNTPs). The cDNAs were analyzed by electrophoresis under denaturing conditions in formamide-polyacrylamide gels. Discrete size products were detected, ranging from 65 to 650 nucleotides-that is, up to the full length of the mRNA template. Increasing the concentrations of all four dNTPs stimulated formation of full-length transcripts and made the incomplete copies less abundant. Hybridization and nuclease digestion experiments indicated that the full-size product is indeed a complete transcript of globin mRNA. Similar results were obtained with chorion mRNAs. The possible usefulness of the discrete partial transcripts is discussed.
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