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  • Title: The N-acetylhexosaminidase components of the ram testis and epididymis.
    Author: Bullock S, Winchester B.
    Journal: Biochem J; 1973 Jul; 133(3):593-9. PubMed ID: 4733242.
    Abstract:
    Three and four N-acetylhexosaminidase components, from ram testis and epididymis respectively, have been separated by ion-exchange chromatography on DEAE-cellulose. Although they all have the same molecular weight (approx. 140000) and very similar catalytic properties towards the synthetic substrates, 4-methylumbelliferyl N-acetyl-beta-glucosaminide and N-acetyl-beta-galactosaminide, isoelectric focusing of the individual components showed that each had a distinct pI value. Isoelectric focusing has also been used to demonstrate the occurrence of multiple forms in ejaculated ram semen.
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