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  • Title: A method for the simultaneous isolation of factor X and prothrombin from bovine plasma.
    Author: Esnouf MP, Lloyd PH, Jesty J.
    Journal: Biochem J; 1973 Apr; 131(4):781-9. PubMed ID: 4737324.
    Abstract:
    1. A method is described for the simultaneous isolation of both Factor X and prothrombin from bovine plasma. The proteins are adsorbed on and eluted from barium sulphate and chromatographed on DEAE-Sephadex and are finally purified by rechromatography on DEAE-Sephadex. 2. The proteins can be purified in 48h from the collection of the blood and the method can be used to process large volumes of plasma. 3. The prothrombin has a molecular weight of 70300; the Factor X, on the other hand, is polydisperse, with most of the protein (86%) having a molecular weight of 56000.
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