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  • Title: Automated analysis of hydroxyproline with elimination of non-specific reacting substances.
    Author: Verch RL, Wallach S, Peabody RA.
    Journal: Clin Chim Acta; 1979 Aug 15; 96(1-2):125-30. PubMed ID: 476953.
    Abstract:
    A modified urinary hydroxyproline method is presented which utilizes an automated technique, Technicon instrumentation and Ehrlich's reagent. Acid hydrolysates are neutralized automatically. Non-specific reacting substances are determined simultaneously with each sample and subtracted as a blank. This method has been found to be superior to the use of charcoal-resin as an absorbent. After blank correction, the mean hydroxyproline excretion of normal and osteoporotic patients are similar. In all groups studied, including Paget's disease, the blanks reresent a significant fraction of total reacting substances and show wide variation between samples. Multiple samples from patients over a two year period show little variation in hydroxyproline excretion when corrected for non-specific reacting substances.
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