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  • Title: The fractionation of adenosine-rich oligoribonucleotides on polyethyleneimine-cellulose.
    Author: Crossley J, Mansbridge J, Williamson R.
    Journal: Biochem J; 1974 Feb; 137(2):299-302. PubMed ID: 4824212.
    Abstract:
    After combined pancreatic and T(1) ribonuclease treatment of RNA, a characteristic series of products of the type A(n)N are obtained, where N can be any of the four ribonucleosides. Depending on whether phosphatase treatment is used before the addition of labelled phosphate at the 5'-terminus with bacteriophage phosphokinase, the labelled oligonucleotides obtained may or may not possess a phosphate group at the 3'- as well as the 5'-end. The behaviour of these characteristic products after electrophoresis on cellulose acetate strips followed by chromatography on polyethyleneimine-cellulose thin-layer plates was examined.
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