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  • Title: Separation of prostaglandin A2 and prostaglandin B2 by ion-exchange liquid chromatography.
    Author: Morozowich W.
    Journal: J Pharm Sci; 1974 May; 63(5):800-2. PubMed ID: 4830010.
    Abstract:
    2 closely related prostaglandins (PGs), A2 and B2, were separated by ion-exchange liquid chromatography. PGA2 and PGB2 are an isomeric pair of PGs which show little resolution on thin-layer chromatography; the pair can be resolved as gas-liquid chromatography, but use of this method requires protection of the C9-carbonyl group. Ion-exchange liquid chromatography, in contrast, requires no protective derivatization to achieve separation of PGA2 and PGB2 from PGE2. 2 different column types were used, and their results compared favorably. In 1 experiment, a triethylaminoethyl cellulose column resolved the 2 species; in another experiment, a strong anion-exchange pellicular support was successful. Both columns were stable and could be used to monitor the stability of PGE2 by following the appearance of PGA2 and PGB2, using the peak height method (i.e., a plot of micrograms injected vs. peak height). Triethylaminoethyl cellulose gave somewhat better resolution of PGA2 and PGB2 as compared with the pellicular strong anion-exchange column, but the advantage of this effect is offset by the tediousness of packing columns of triethylaminoethyl cellulose.
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