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  • Title: Analysis of melibiose mutants deficient in alpha-galactosidase and thiomethylgalactoside permease II in Escherichia coli K-12.
    Author: Schmitt R.
    Journal: J Bacteriol; 1968 Aug; 96(2):462-71. PubMed ID: 4877127.
    Abstract:
    Three types of mutants (mel(-)) unable to metabolize the alpha-d-galactoside, melibiose, were derived from Escherichia coli K-12. One type lacked alpha-galactosidase; another lacked a specific transport system, termed thiomethylgalactoside (TMG) permease II; and the third lacked both of these functions. The mutational sites were genetically mapped by recombination frequency with different markers and by determination of chromosomal transfer in interrupted-mating experiments. All three mel(-) mutant types mapped in a cluster near to the metA marker on the E. coli chromosome and were cotransducible. Induction studies revealed that the three alpha-d-galactosides, melibiose, melibiitol, and galactinol, induced alpha-galactosidase and TMG permease II coordinately; d-galactose also induced them but only in a galactokinaseless mutant. These data suggest that alpha-galactosidase and TMG permease II may be components of a common operon.
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