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  • Title: Single crystals of transfer RNA from formylmethionine and phenylalanine transfer RNA's.
    Author: Hampel A, Labanauskas M, Connors PG, Kirkegard L, RajBhandary UL, Sigler PB, Bock RM.
    Journal: Science; 1968 Dec 20; 162(3860):1384-7. PubMed ID: 4880853.
    Abstract:
    Reproducible conditions have been developed for crystallization of transfer RNA. The conditions may be applicable to many pure transfer RNA species since identical procedures (except for initial transfer-RNA concentration) yielded good crystals from both yeast and Escherichia coli transfer RNA. These crystals, which must be kept at temperatures below about 10 degrees C and handled in vapor of controlled alcohol concentration, have been studied by x-ray crystallography. The availability of crystals of a nucleic acid opens a route for extending knowledge of the tertiary structure of transfer RNA and its relation to important biological functions.
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