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  • Title: A comparison of the initiation factors of eukaryotic protein synthesis from ribosomes and from the postribosomal supernatant.
    Author: Thomas A, Goumans H, Amesz H, Benne R, Voorma HO.
    Journal: Eur J Biochem; 1979 Aug 01; 98(2):329-37. PubMed ID: 488105.
    Abstract:
    A purification procedure is described for the initiation factors of protein synthesis from rabbit reticulocytes: (a) from the ribosomal wash and (b) from the postribosomal supernantant. A comparison is made between these preparations with respect to yield and specific activity. eIF-4A and eIF-4D occur mainly in the postribosomal supernatant; eIF-2, eIF-4C and eIF-5 are more evenly divided over both fractions, whereas eIF-1, eIF-3 and eIF-4B are found almost exclusively in the ribosomal wash. No significant difference in specific activity could be detected when factors from both sources were compared, with a possible exception of eIF-4A and eIF-4D.
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