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  • Title: Infection of tobacco mesophyll protoplasts by tobacco mosaic virus.
    Author: Takebe I, Otsuki Y.
    Journal: Proc Natl Acad Sci U S A; 1969 Nov; 64(3):843-8. PubMed ID: 4905990.
    Abstract:
    It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus. The infection occurred when purified tobacco mosaic virus particles were added to a protoplast suspension in the presence of poly-L-ornithine. The virus multiplied in these protoplasts to a level of 10(6) virus particles per infected protoplast during 24 hours of incubation. The efficiency of infection was remarkably high, exceeding that by mechanical inoculation of tobacco leaves.
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