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  • Title: Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II.
    Author: Kornberg T, Gefter ML.
    Journal: Proc Natl Acad Sci U S A; 1971 Apr; 68(4):761-4. PubMed ID: 4927672.
    Abstract:
    The major DNA-synthesizing enzyme present in Pol A(1) (-)Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg(++), NH(4) (+), and native DNA for maximal activity. The enzyme activity is sensitive to sulfhydryl reagents and is insensitive to anti-DNA polymerase I antiserum. A second DNA-synthesizing enzyme, present in low amounts, has been identified in Pol A(1) (-) extracts. The relationship of this enzyme to DNA polymerases I and II is discussed.
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