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  • Title: Regulation of IgM memory expression by spleen cells cultured in diffusion chambers.
    Author: Borella L.
    Journal: Immunology; 1971 Mar; 20(3):289-98. PubMed ID: 4927928.
    Abstract:
    In intact mice IgM memory to sheep erythrocytes (SRBC) has an early peak followed by a declining phase. The aim of this study was to determine whether the decay of IgM memory is regulated by the early appearance of cells producing antibodies of IgG classes. The expression of IgM memory was studied in SRBC-primed mouse spleen cells restimulated with SRBC in Millipore diffusion chambers implanted in irradiated hosts. Restimulation of spleen cells obtained from mice primed with SRBC 2 days before the beginning of the culture (D2 cells) induced a ten-fold increase in IgM PFC above the number expected in cultures of non-primed cells. The number of IgM PFC significantly decreased in cultures of cells obtained 4 days after priming (D4 cells). Inhibition of IgM PFC occurred in cultures of D2 cells co-cultured with D4 cells in single or double compartment diffusion chambers. In primed spleen cells cultured in double compartment chambers there was an inverse relationship between the number of early IgG PFC in one side and appearance of IgM PFC in the other. A 500-fold increase in antigen concentration in chambers containing D4 cells induced a slow, but consistent, rise in IgM PFC. The data indicate the expression of IgM memory in this culture system is inhibited by the appearance of cells producing antibody of IgG classes as early as 4–10 days after primary antigenic stimulation. It is postulated that a similar process may also regulate the kinetics of IgM memory decay in the intact animal.
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