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  • Title: Membrane association of conjugally transferred deoxyribonucleic acid in Escherichia coli minicells.
    Author: Shull FW, Fralick JA, Stratton LP, Fisher WD.
    Journal: J Bacteriol; 1971 May; 106(2):626-33. PubMed ID: 4929870.
    Abstract:
    The conjugally acquired deoxyribonucleic acid (DNA) of small, anucleate cells ("minicells") of a mutant strain of Escherichia coli K-12 was found to be predominantly associated with the bacterial membrane. Evidence from X-irradiation studies in vivo shows that there is no decrease in DNA-membrane association under conditions which reduce the DNA to one-sixth its original size and suggests the possibility of multiple DNA-membrane association sites. Preliminary enzymatic studies indicate the involvement of protein, DNA, and lipids in the membrane association of the DNA.
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