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  • Title: The relationship of antibody-forming cells to rosette-forming cells.
    Author: Wilson JD.
    Journal: Immunology; 1971 Aug; 21(2):233-45. PubMed ID: 4937051.
    Abstract:
    Rosette-forming cells (RFC) increased in numbers in the spleens of mice following injection of sheep red blood cells (SRBC). Very sensitive assay system for detecting plaque-forming cells (PFC) showed that RFC and PFC were present in approximately equal numbers at the height of the immune response. Thereafter PFC numbers declined much more rapidly than RFC. Two techniques were used to study the contribution of PFC to RFCs (a) velocity sedimentation through foetal calf serum gradients and (b) transfer of individual RFC by micromanipulator into the PFC assay gel containing complement and rabbit antimouse IgG antiserum to identify what proportion of RFC were secreting antibody. It was shown that, when rosettes were prepared at 4°, <10 per cent were formed by PFC at 4 days after immunization, and <2 per cent at 6 days. Rosettes prepared at 37° contained up to 16 per cent PFC. It was concluded that PFC had either no cell bound antigen-binding receptors or that the receptors were not demonstrable at 4° by the particular rosette preparation used in the study. Rosettes prepared late in the immune response were more resistant to mechanical agitation than those prepared early in the response.
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