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  • Title: The cell walls of Pseudomonas aeruginosa. General composition.
    Author: Clarke K, Gray GW, Reaveley DA.
    Journal: Biochem J; 1967 Nov; 105(2):749-54. PubMed ID: 4967073.
    Abstract:
    1. Cell walls of Pseudomonas aeruginosa were prepared and analysed. 2. Separate preparations were found to be reproducible, e.g. the phosphorus contents of all batches lay in the range 2.0-2.1%. 3. Ninhydrin-positive compounds in hydrolysates accounted for 43-46% of the cell wall and 79-87% of the nitrogen of the cell wall. Examination of the results for individual ninhydrin-positive compounds showed that 5-15% of the cell wall was murein and about 30% protein. 4. Trypsin treatment of crude cell-wall preparations preferentially liberated arginine, lysine and leucine, but it was not clear whether these arose from cell-wall or cytoplasmic proteins.
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