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  • Title: The stability of methylated purines and of methylphosphotriesters in the DNA of V79 cells after treatment with N-methyl-N-nitrosourea.
    Author: Warren W, Crathorn AR, Shooter KV.
    Journal: Biochim Biophys Acta; 1979 Jun 20; 563(1):82-8. PubMed ID: 497216.
    Abstract:
    V79-379A cells growing in suspension culture were treated with N-methyl-N-nitrosourea at concentrations of 0.6 and 1.2 mM. After incubation for periods from 1 to 48 h DNA was isolated from the cells and the concentrations of 7-methylguanine, O6-methylguanine, 3-methyladenine and methyl phosphotriesters were determined. After correction for dilution resulting from DNA synthesis during the incubation it was found that no loss of O6-methylguanine or methylphosphotriesters occurred; 7-methylguanine disappeared with a half-life of 22 h and 3-methyladenine was detectable only immediately after the initial treatment. The results show that these cells eliminate 7-methylguanine and 3-methyladenine from DNA by a repair process but are unable to excise or repair O6-methylguanine or methyl phosphotriesters.
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