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  • Title: [Secondary structure of RNA secreted by Ehrlich ascites carcinoma cells].
    Author: Agliullina DG, Estulina LA, Vinter VG.
    Journal: Biokhimiia; 1979 Aug; 44(8):1409-15. PubMed ID: 497286.
    Abstract:
    The RNA, secreted by the cells of Ehrlich ascite carcinoma stimulates the inoculability and growth of the tumour. It contains double-helical regions, is resistable to the effects of pancreatic RNAse, has a melting point at 74 degrees and is eluted from the hydroxylapatite column by 0,25 M phosphate buffer. During its interaction with ethidium bromide the RNA increases the fluorescence of the dye. The amount of double-helical regions in the RNA makes up to 60%. These double-helical regions are formed in the carcinoma-secreted RNA due to RNA self-supercoiling. This was demonstrated by fluorescence studies of the RNA-ethidium bromide complex under various RNA denaturation and renaturation conditions.
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