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  • Title: Comparison between immunofixation and crossed immunoelectrophoresis for the detection of C3 activation products.
    Author: De Vecchi A, Montagnino G, Massaro L, Constantino A, Bencini PL, Tarantino A.
    Journal: Clin Chim Acta; 1979 Sep 15; 97(1):27-32. PubMed ID: 498518.
    Abstract:
    C3 breakdown products were measured in 51 fresh and stored sera and/or EDTA plasma samples from 18 healthy subjects, 8 patients affected by essential mixed cryoglobulinaemia, and 15 patients with miscellaneous glomerulonephritis, by simultaneous crossed immunoelectrophoresis and immunofixation. C3 splitting products, as determined by both methods compared well, and showed a highly significant correlation. The advantages and reliability of these two methods are discussed. Immunofixation seems to be the most suitable for routine use in clinical practice, being less expensive and more rapid to perform.
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