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  • Title: Cell wall binding properties of the Bacillus subtilis autolysin(s).
    Author: Fan DP.
    Journal: J Bacteriol; 1970 Aug; 103(2):488-93. PubMed ID: 4988245.
    Abstract:
    Cell walls isolated from exponentially growing Bacillus subtilis have autolysin(s) attached to them. An autolysin can be released from the walls by incubation at 0 C with 3 m LiCl. The enzyme can reattach to walls when the salt concentration is reduced. The bound enzyme cannot be removed or destroyed by washing the walls with 8 m urea at 0 C. The binding of free enzyme to walls at 0 C can take place normally in the presence of 2 m urea.
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