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  • Title: A rapid plastic embedding technique for preparation of three-micron thick sections of decalcified hard tissue.
    Author: Kimmel D, Jee WS.
    Journal: Stain Technol; 1975 Mar; 50(2):83-6. PubMed ID: 49947.
    Abstract:
    A 24 hour start-to-finish method is described for the preparation of three-micron-thick sections of decalcified hard tissues. Following acetone dehydration, the tissue to be embedded is infiltrated under vacuum with a series of graded clearing solutions which approach the content of the final methyl methacrylate mixture. After overnight in a 35 C oven, the plastic is polymerized by four hours heating at 42 C. Three-micron-thick sections are then easily prepared by using a Jung microtome for high resolution histologic or detailed autoradiographic procedures.
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