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  • Title: Immunoglobulin G and free kapa-chain synthesis in different clones of a hybrid cell line.
    Author: Mohit B.
    Journal: Proc Natl Acad Sci U S A; 1971 Dec; 68(12):3045-8. PubMed ID: 5002282.
    Abstract:
    13 clonal cell lines were isolated from a hybrid cell population established by cell fusion between cloned BALB/c myeloma cells that were resistant to 8-azaguanine and produced immunoglobulin G and free kappa chain and C(67)BL/6n lymphoma cells that were resistant to bromodeoxyuridine and did not produce immunoglobulins. Some of the histocompatibility-2 antigens of both parental cell lines could be demonstrated on all of the hybrid clones. 11 clones synthesized only free kappa chain. Two clones synthesized IgG of BALB/c type, as well as free kappa chain. These clones had higher chromosome number than did the clones that synthesized only kappa chains.
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