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  • Title: Production of L-arginine by arginine hydroxamate-resistant mutants of Bacillus subtilis.
    Author: Kisumi M, Kato J, Sugiura M, Chibata I.
    Journal: Appl Microbiol; 1971 Dec; 22(6):987-91. PubMed ID: 5002904.
    Abstract:
    l-Arginine hydroxamate inhibited the growth of various bacteria, and the inhibition was readily reversed by arginine. l-Arginine hydroxamate (10(-3)m) completely inhibited the growth of Bacillus subtilis. This inhibitory effect was prevented by 2.5 x 10(-4)ml-arginine, which was the most effective of all the natural amino acids in reversing the inhibition. l-Arginine hydroxamate-resistant mutants of Bacillus subtilis were isolated and found to excrete l-arginine in relatively high yields. One of the mutants, strain AHr-5, produced 4.5 mg of l-arginine per ml in shaken culture in 3 days.
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