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  • Title: Hybridization of pigeon globin messenger RNA with complementary DNA synthesized in vitro by reverse transcription: influence of the homopolymeric regions.
    Author: Gasaryan KG, Tarantul VZ, Baranov YN, Frolova LY, Kisselev LL.
    Journal: Nucleic Acids Res; 1975 Jul; 2(7):1203-12. PubMed ID: 50588.
    Abstract:
    The kinetics of hybridization of pigeon globin messenger RNA with complementary cDNA synthesized by means of AMV reverse transcriptase is complex. Addition of poly A or poly U in excess to the reaction mixture normalized the kinetics. It is concluded that association of the complementary homopolymeric regions of mRNA and cDNA accelerates the complex formation between heteropolymeric sequences in a fraction of the molecules.
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