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  • Title: RNA synthesis in newly isolated and cultivated mesophyll protoplasts.
    Author: Paszkowski J, Kleczkowski K.
    Journal: Acta Biochim Pol; 1979; 26(1-2):5-10. PubMed ID: 506617.
    Abstract:
    Tobacco leaf mesophyll protoplasts exhibit low incorporation of [3H]uridine and 32P into RNA, up to 12 h of cultivation, irrespective of the presence of phytohormones. After 24 h of cultivation a dramatic increase in RNA synthesis is observed; it is the highest in the heterogeneous nucleoplasmic RNA fraction. The protoplasts cultivated in the absence of phytohormones show lower incorporation of precursors.
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