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  • Title: [Value of low ionic-strength salt solution in indirect antiglobulin tests with erhythrocytes].
    Author: Walewska I, Kuśnierz G.
    Journal: Acta Haematol Pol; 1979; 10(3):159-68. PubMed ID: 506654.
    Abstract:
    A series of comparative investigations were carried out for estimation of the value of the indirect antiglobulin test (IAT) in the modification of Löw and Messeter in which a suspension of erythrocytes in low ionic-strength salt solution called by the authors LISS, is used for incubation of erythrocytes with tested serum, that is in phase I of this test. The results of these experiments demonstrated a significant superiority of this modification over the conventional techniques, particularly over the tile technique used generally in this country. The IAT-LISE method allows not only phase I to be reduced from 60 to 20 minutes, but increasing the intensity of the reaction it facilitates also detection of blood group antibodies and extends the range of their discovery, which is of particular importance in the compatibility tests before blood transfusion. These results were a basis for routine application of this method nad it was concluded that the IAT-LISS modification should be introduced widely in the Polish serological laboratories. Attention is called to a number of technical details important to obtain reproducible and une quivocal results.
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