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  • Title: [Study of heterogeneity of chromatin endonuclease fragments, using fractionation by step-wise endonucleolysis].
    Author: Kharchenko EP, Nalivaeva NN, Kolesnikova EIu, Fedotova EA.
    Journal: Biokhimiia; 1979 Oct; 44(10):1768-75. PubMed ID: 508854.
    Abstract:
    Isolated nuclei from rat liver were incubated at different time intervals under conditions, optimal for manifestation of the Ca, Mg-dependent endonuclease activity. After each of the 6 endonucleolyses chromatin was extracted and 6 chromatin fractions (I--VI) and "residual" chromatin were collected. A comparative analysis of the "early" (I--III) and "late" chromatin fractions revealed an increased RNA content in the "late" fractions and changes in the composition of the non-histone proteins. Electrophoresis in acrylamide gel concentration gradient demonstrated that fractions I--III predominantly contain high molecular weight fragments whereas fractions IV-VI are represented by highly fragmented chromatin. Each fraction was characterized by peculiar shapes of alkaline denaturation curves and by heterogeneity of charges of their constituent chromatin fragments.
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