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  • Title: Radioimmunoassay for serum medroxyprogesterone acetate in Thai women receiving injectable DMPA contraceptive.
    Author: Werawatgoompa S, Pongpradit T, Leepipatpaiboon S, Sukanthanak A.
    Journal: Contraception; 1979 Oct; 20(4):319-27. PubMed ID: 509958.
    Abstract:
    This study aims to: 1) develop a sensitive and accurate radioimmunoassay (RIA) for medroxyprogesterone acetate (MPA); 2) correlate serum MPA concentration with ovarian hormones; and 3) determine whether ovulation, by using 100 mg MPA, is inhibited to the same degree as with the conventional intramuscular injection of 150 mg. MPA. Using goat antibodies made against bovine serum albumin conjugate of 3-(O-carboxymethyl) oxine MPA, an RIA for serum MPA was developed. The method had an accuracy between 71.4-116%; precision of the assay in terms of coefficient of variation was between 7.6-8.84%. The method was used on 6 healthy Thai women 20-35 years of age who had been sterilized before the study. 100 mg. MPA were administered intramuscularly on day 5 of the menstrual cycle; blood samples were collected every 5 days from day 10 to day 90. At day 10 post-injection, MPA levels ranged from 1 to 4.83 ng/ml, decreasing gradually, with little fluctuation, during the study period. In 5 subjects (K, M, N, P and T), serum MPA levels gradually declined and stabilized at about 0.1 to 1.5 ng/ml, while in another subject (U), serum MPA level gradually declined to very low levels (0.84 to 0.02 ng/ml after 60 days posttreatment). In subject U, raised progesterone level ( 4 ng/ml) was observed on days 65 and 90, suggesting a return of normal ovarian cyclicity. The findings showed that ovulation failed to occur within the 90-day observation period in 5 of the 6 subjects who were given 100 mg. of intramuscular MPA injections. As it is not definitely known when the ovulation of these 5 women occurred, this study cannot be compared with the findings of the other studies.
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