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  • Title: Lymphocyte surface components. I. Stimulation of enzyme-treated rabbit lymphocytes by non-specific mitogens.
    Author: Goodall DM, Pardoe GI, Gregory JK.
    Journal: Clin Exp Immunol; 1971 Nov; 9(5):645-55. PubMed ID: 5145431.
    Abstract:
    Rabbit lymphocytes from various locations were treated briefly with proteases and the rate of incorporation of thymidine following stimulation by `non-specific' stimulants (Staphylococcus filtrate, SF, and Phaseolus vulgaris phytohaemagglutinin, PHA) measured. The proteases themselves, especially at concentrations exceeding 0·005%, sometimes caused cells to incorporate more thymidine than untreated cells. Stimulation with SF or PHA was much greater when lymphocytes were first treated with protease at low concentrations (when enzyme alone failed to stimulate). This effect was shown particularly by lymphocytes from gut-associated lymphoid organs. Peripheral blood lymphocytes, while unaffected by proteases at a concentration of 0·005%, incorporated increased amounts of thymidine after treatment with proteases at higher concentrations: pronase is especially potent. Further evidence is presented, distinguishing between the cytoagglutinating and mitogenic characters of PHA.
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