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  • Title: Screening method for vitamins A and D in fortified skim milk, chocolate milk, and vitamin D liquid concentrates.
    Author: Henderson SK, McLean LA.
    Journal: J Assoc Off Anal Chem; 1979 Nov; 62(6):1358-60. PubMed ID: 521421.
    Abstract:
    Vitamin A was determined in fortified chocolate milk and skim milk; vitamin D was determined in fortified chocolate milk, skim milk, and vitamin D concentrates, using reverse phase high pressure liquid chromatography (HPLC). The sample is saponified, extracted with hexane, and chromatographed in an HPLC system on a 10 micron Vydac TP reverse phase C18 column, using acetonitrile-methanol (9+1) as the mobile phase. For 6 replicates, the recoveries of vitamins A and D, using this procedure, were 99 and 98%, respectively.
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