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  • Title: Photohydration of uridine in the RNA of coliphage R17. II. The relationship between ultraviolet inactivation and uridine photohydration.
    Author: Remsen JF, Miller N, Cerutti PA.
    Journal: Proc Natl Acad Sci U S A; 1970 Feb; 65(2):460-6. PubMed ID: 5263776.
    Abstract:
    Uridine photohydrates (6-hydroxy-5,6-dihydrouridine) are the major products formed upon irradiation of the RNA bacteriophage R17 with ultraviolet light at 280 mmu, while cyclobutane-type pyrimidine dimerization does not occur to an appreciable extent. The suppression of dimerization may indicate that the RNA inside the phage is held in a rigid conformation in close contact with the phage protein. A value of 0.94 (+/-0.10) uridine photohydrates per biological hit is obtained from a correlation of the fraction of phage surviving with the number of photohydrates formed as a function of ultraviolet dose. It is concluded that uridine photohydrates represent a major part of the lethal damage caused by ultraviolet light in bacteriophage R17. An efficient system for dark repair of uridine photohydration seems to be lacking in E. coli.
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