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  • Title: Effect of anti-rabbit IgG antisera on immune haemolysis.
    Author: Kimura I, Eulitz M, Thierfelder S.
    Journal: Immunology; 1970 Nov; 19(5):689-98. PubMed ID: 5312471.
    Abstract:
    Initiation of immune haemolysis was inhibited by dog and sheep anti-rabbit IgG antisera and the further advance of the already-initiated complement fixation reaction was also inhibited unless it had proceeded to the state of EAC142. The hindrance of C1 uptake in the former case and the elution of bound C1 in the latter were proved to be the mechanisms by kinetic studies and the detection of released C1 in the supernatant of antiserum-treated intermediate cells. It can be said the haemolysin influences its effect up to the C2 fixation step by means of the C1 bound on it. Anti-guinea-pig C4 antiserum also inhibited intermediate cells but its effect was different from that of anti-rabbit IgG antiserum since the release of C1 could not be detected in this case.
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