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  • Title: [A fast and simple micromethod for estimating proteins].
    Author: Sydow G.
    Journal: Acta Biol Med Ger; 1979; 38(8):1141-4. PubMed ID: 532491.
    Abstract:
    The assay is based on the property of proteins to form a complex with bromophenol blue. In alkaline solution this complex shows an intensive blue colour which is well measurable at 598 nm. The method is especially useful for small volumes (5-50 microliter) with a content of 5-30 micrograms of protein. By modification the test can be also used for the measurement of higher amounts of protein.
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