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  • Title: Analytical studies of maridomycin. II. Separation of 9-propionylmaridomycins by thin-layer chromatography.
    Author: Kondo K.
    Journal: J Chromatogr; 1979 Feb 01; 169():337-42. PubMed ID: 536424.
    Abstract:
    Priopionyl derivatives of maridomycins, 9-propionylmaridomycins (PMDMs), are sixteen-membered ring macrolide antibiotics of six analogous components: I, II, III, IV, V and VI. The present paper deals with the separation and quantitative analysis of these components. The analysis was performed by thin-layer chromatographic separation, addition reaction of gaseous iodine with PMDMs on the plate, extraction of the PMDM-iodine complexes, and subsequent analysis of the amount of reacted iodine, using an automatic analysis system. To ascertain the reaction product of this system, PMDM III-iodine complex was synthesized separately under a liquid-phase reaction and the ratio of PMDM III and iodine atoms was determined to be 1:3 on physicochemical examination.
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