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  • Title: [Biosynthesis of citric and isocitric acids by the wild-type and mutant strains of Candida lipolytica in media containing different carbon sources].
    Author: Finogenova TV, Grinchak AV, Illarionova VI, Shishkanova NV.
    Journal: Prikl Biokhim Mikrobiol; 1979; 15(6):811-6. PubMed ID: 538009.
    Abstract:
    Experiments were carried out to examine the capacity of two strains of Candida lipolytica, producing citric and isocitric acids in the alkane and glucose containing media, to grow on different two- and three-carbon compounds. The strains did not grow on oxalate, glyoxalate, glycolate, malonate or propionate. When cultivated in the media containing acetate, ethanol, glycerol, glucose or hexadecane, supersynthesis of the acids started after complete consumption of the nitrogen source and resultant delay of the culture growth. Either strain discharged the two acids in a proportion that depended on the strain nature and the type of the carbon source. The mutant strain produced only citrate while the wild-type synthesized both citrate and isocitrate, the ratio of which was related to the nature of the carbon source utilized.
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